Product Info Summary
SKU: | PB9169 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD79b Antibody Picoband™
SKU/Catalog Number
PB9169
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD79b Antibody Picoband™ catalog # PB9169. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD79b Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9169)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD79b recombinant protein (Position: A29-E229). Human CD79b shares 70% amino acid (aa) sequence identity with mouse CD79b.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9169 is reactive to CD79B in Human, Mouse, Rat
Applications
PB9169 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
35 kDa
Calculated molecular weight
26.048kDa
Background of CD79B
CD79b molecule, immunoglobulin-associated beta, also known as CD79B (Cluster of Differentiation 79B), is a human gene. By fluorescence in situ hybridization, It is mapped to 17q23.3. The CD79B protein together with the related CD79A protein, forms a dimer associated with membrane bound immunoglobulin in B-cells, thus forming the B-cell antigen receptor (BCR) which is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). CD79b also can enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD79B using anti-CD79B antibody (PB9169).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human CD79B protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD79B antigen affinity purified polyclonal antibody (Catalog # PB9169) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD79B at approximately 45 kDa. The expected band size for CD79B is at 45 kDa.
Click image to see more details
Figure 2. Western blot analysis of CD79B using anti-CD79B antibody (PB9169).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Hl-60 whole cell lysates,
Lane 3: human HUT whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD79B antigen affinity purified polyclonal antibody (Catalog # PB9169) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD79B at approximately 35 kDa. The expected band size for CD79B is at 26 kDa.
Click image to see more details
Figure 3. IHC analysis of CD79B using anti-CD79B antibody (PB9169).
CD79B was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD79B Antibody (PB9169) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of CD79B using anti-CD79B antibody (PB9169).
CD79B was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD79B Antibody (PB9169) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of CD79B using anti-CD79B antibody (PB9169).
CD79B was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD79B Antibody (PB9169) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IF analysis of CD79B using anti-CD79B antibody (PB9169).
CD79B was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CD79B Antibody (PB9169) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 7. IF analysis of CD79B using anti-CD79B antibody (PB9169).
CD79B was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CD79B Antibody (PB9169) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CD79B (Source: Uniprot.org, NCBI)
Gene Name
CD79B
Full Name
B-cell antigen receptor complex-associated protein beta chain
Weight
26.048kDa
Alternative Names
B29; B29B-cell-specific glycoprotein B29; B-cell antigen receptor complex-associated protein beta chain; CD79B antigen (immunoglobulin-associated beta); CD79b antigen; CD79b molecule, immunoglobulin-associated beta; CD79B; IGB; IGBAGM6; Ig-beta; Immunoglobulin-associated B29 protein CD79B AGM6, B29, IGB CD79b molecule B-cell antigen receptor complex-associated protein beta chain|B-cell-specific glycoprotein B29|CD79b antigen (immunoglobulin-associated beta)|CD79b molecule, immunoglobulin-associated beta|Ig-beta|immunoglobulin-associated B29 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD79B, check out the CD79B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD79B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD79b Antibody Picoband™ (PB9169)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CD79b Antibody Picoband™
Question
We have been able to see staining in rat small intestine spleen. Do you have any suggestions? Is anti-CD79b antibody supposed to stain small intestine spleen positively?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
From what I have seen in literature small intestine spleen does express CD79B. From what I have seen in Uniprot.org, CD79B is expressed in spleen, small intestine spleen, lymph, among other tissues. Regarding which tissues have CD79B expression, here are a few articles citing expression in various tissues:
Lymph, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-10
Question
Our team were content with the WB result of your anti-CD79b antibody. However we have seen positive staining in lymph cell membrane using this antibody. Is that expected? Could you tell me where is CD79B supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Based on literature, lymph does express CD79B. Generally CD79B expresses in cell membrane. Regarding which tissues have CD79B expression, here are a few articles citing expression in various tissues:
Lymph, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-23
Question
Our lab used your anti-CD79b antibody for IF on spleen in a previous experiment. I am using human, and We want to use the antibody for IHC-P next. I would like examining spleen as well as lymph in our next experiment. Could give a recommendation on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
I have checked the website and datasheets of our anti-CD79b antibody and it appears that PB9169 has been tested on human in both IF and IHC-P. Thus PB9169 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-20
Question
My lab would like using your anti-CD79b antibody for antigen activates b cell receptor (bcr) leading to generation of second messengers studies. Has this antibody been tested with western blotting on hut whole cell lysate? We would like to see some validation images before ordering.
C. Krishna
Verified customer
Asked: 2014-12-19
Answer
I appreciate your inquiry. This PB9169 anti-CD79b antibody is validated on human tonsil tissue, raji whole cell lysate, hut whole cell lysate, mouse spleen tissue, rat spleen tissue. It is guaranteed to work for IF, IHC-P, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-12-19
Question
We are currently using anti-CD79b antibody PB9169 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
M. Zhao
Verified customer
Asked: 2013-10-08
Answer
The anti-CD79b antibody (PB9169) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-10-08